Distinguishing Rose Cultivars by Polyacrylamide Gel Electrophoresis. I. Extraction and Storage of Protein and Active Enzymes from Rose Leaves1

Abstract

Abstract Procedures were developed to extract protein and active enzymes from rose leaves. A suitable extraction medium, extraction time, and sample application rate was determined for each system in which bands could be separated. Sixteen enzyme systems and anionic protein were investigated, with only anionic protein, peroxidase, esterase, malate dehydrogenase, cytochrome oxidase, phenoloxidase, and polyphenoloxidase producing clear bands. Existing staining techniques were adequate for staining cytochrome oxidase, phenoloxidase, and polyphenoloxidase activity, but modifications of the reported anionic protein, peroxidase, esterase and malate dehydrogenase stains were required. Isoenzyme changes which occur during 0, 9, 12, and 16 weeks storage are discussed.