Plasma exosomal <i>miR-1260a, miR-7977</i> and <i>miR-192-5p</i> as diagnostic biomarkers in epithelial ovarian cancer

Abstract

<b>Aim:</b> The study aimed to clarify the diagnostic value of exosomal miRNAs in epithelial ovarian cancer (EOC). <b>Methods:</b> Plasma exosomes were isolated from peripheral blood of EOC patients and healthy donors by ultracentrifugation and verified by transmission electron microscopy, qNano and western blot. The expression of exosomal miRNAs was detected by quantitative PCR, and the diagnostic efficiency of exosomal miRNAs was evaluated by receiver operating characteristic analysis. <b>Results:</b> Exosomal <i>miR-1260a</i>, <i>miR-7977</i> and <i>miR-192-5p</i> were significantly decreased in EOC as compared with healthy controls. The area under the curve of the combination of three exosomal miRNAs was 0.8337. Moreover, the level of exosomal <i>miR-7977</i> was related to the neutrophil-lymphocyte ratio, which decreased in EOC patients with a high neutrophil-lymphocyte ratio. <b>Conclusion:</b> Exosomal <i>miR-1260a</i>, <i>miR-7977</i> and <i>miR-192-5p</i> act as potentially EOC diagnostic and prognostic biomarkers.