Clonal Dissemination of Aeromonas hydrophila With Binary Carriage of blaKPC-2-Bearing Plasmids in a Chinese Hospital

Abstract

Dissemination of the Klebsiella pneumoniae carbapenemase (KPC)-encoding gene among Enterobacterales is common but relatively rare in Aeromonas spp. In this study, we characterized two KPC-2-producing Aeromonas hydrophila strains (Ah2101 and Ah2111), each isolated from a patient in different intensive care units (ICUs) of a Chinese hospital. Whole-genome sequencing (WGS) revealed simultaneous carriage of the bla KPC−2 and imiH genes, both of which encode high-level carbapenem resistance in these two A. hydrophila isolates. The two isolates were shown to be clonally related and each isolate harbored two distinguishable bla KPC−2 -bearing plasmids, only one of which was transferrable to A. hydrophila , but not Escherichia coli EC600 via conjugation. The genetic element that contains bla KPC−2 in these two plasmids, namely IS Kpn27 -Δ bla TEM−1 - bla KPC−2 -IS Kpn6 , was structurally identical, commonly detected in Enterobacterales, and associated with Tn 3 -based transposons. In addition, more than sixty putative genes that encode various virulence factors were identified in these two A. hydrophila isolates. This is the first study that reports clonal dissemination of carbapenem-resistant A. hydrophila strains carrying structurally different bla KPC−2 -bearing plasmids. Further investigation is warranted to monitor the future transmission of bla KPC−2 -bearing plasmids in A. hydrophila in clinical settings.