Cellular and Molecular Gastroenterology and Hepatology · 2022 · 25 citations · 10 references
Life SciencesCell BiologyImmunologyInnate ImmunityMucosal ImmunologyTissue EngineeringGastrointestinal ImmunologyGastrointestinal InflammationIntestinal DevelopmentMacrophage BiologyMucosal Immune SystemMucosal ImmunityGastrointestinal Tract OrganogenesisIntestinal HomeostasisIntestinal Mucosal ImmunityIntestinal Tissue EngineeringMonocyte-to-macrophage DifferentiationOrganoidsResident Tissue Macrophages
Intestinal macrophages are largely responsible for the innate immune response and also for intestinal homeostasis.1 We have developed novel xenogeneic-free human intestinal organoids (XF-HIOs) that are uniquely structured with an apical-out mucosal epithelium and complex mesenchymal tissue, including smooth muscle and intestinal nerve cells.2,3 To further develop XF-HIOs containing tissue macrophages, we first prepared human-induced pluripotent stem cell (hiPSC)-derived monocyte-like cells (pMCs).
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