Agricultural and Biological Chemistry · 1991 · 27 citations · 0 references
AgricultureBiochemistryFood ScienceFood AnalysisFood ChemistryPlant BiochemistryCereal Grain ChemistryExtraction TechniquesProtein SeparationSeed Nutritional CompositionComposition Of GrainsGrain FractionationGrain Nutritional CompositionGrain Protein ContentPhysicochemical PropertiesProtein BodiesProtein ExtractionSeed Protein Extraction
Two albumin fractions were extracted from defatted flours of seeds of Amaranthm hypochondriacus and A. cmentus. One fraction (Alb-1) was extracted with 0.5M NaCl or water when used as the first solvent. Another fraction (Alb-2) was extracted with water after removing Alb-1 and globulin. Apparent pIs of Alb-1 and Alb-2 were 4.5-7.5 and 5-6, respectively. Alb-2 was precipitated by various metal ions (1mM), while Alb-1 was not. Alb-2 contained four major components (Mr=56, 36, 26, 22 kD), although Alb-1 had no particular major proteins. Pronase treatment of amaranth flour before protein extraction decomposed Alb-1, but not Alb-2, as seen by SDS-PAGE analysis. The findings that Alb-2 was protected from the Pronase treatment and it could not be extracted until Alb-1 and globulin were removed suggests that Alb-2 might be associated with protein bodies [Irving et al., J. Food Sci., 46, 1170 (1981)].