Studies on the colicinogenic factor in Escherichia coli K12.Induction of colicin production by mitomycin C.

Abstract

BIKE\'S JouR\AL 5. 1-n (1962) Studies on the Colicinogenic Factor in Escherichia coli K 12 Inducfion of Colicin Production by Mifomycin CAlitoinycii} Ginduccs thc PIOduction orcolicinin E. coliK 30 and colicinogenic strains or XI2 'The production of colicin sccms to invol\, c it 110.0syntllCSis of protein in a 11'action of the population of a colicinogcnic strain.The proportion of this F1 action is incrcasccl by itcalmcnt \\, ith inducing agents.The optimal condiiions \\, CTC clenTonstraiecl by two methods, lacunae collniing anti assay of Ihc stipernatant.By boni methods 11te optimal conditions \VCI c round 10 lie the same DJp, r!,"e, 11 of Gene!i", Of aka Uni, err!'ty Med^^a!School, Of"Aa (Refei, ed/or pi^611t"!!'on, ,Jan"ary 30, 1962) TEIJI 1111MA* A, Iytomyciil C, aiT antil)lotic isolated from SII',!lion!yceJ carJPi/o5wJ, selectively inhibits the synthesis of deoxyril)onucleic acid (DNA) in EJche!ichid co!i (Shil, a at n/., 1959).Additional studies ITave revealed that DNA synthesis it, hicll has I)CGn Inhil)ited I, y Initomycin C treatmcnt can I)e promptly restored by infection of th, ,, its with bart, "rophag, T2 (S, kigu.hi and Titan 'I, 1959, 1960^).Must of the Iiactcriophagcs \\, hich nttiltiply in the pi'esciice of initomycin C arc IToninfectious (SekigucllI and Takaoi, 1960b), I)ut these I>hages nave the ability of multiplicity reactivation, cross reactivatioil and mutual exclusion (Okub0, 1962).NIItomyciiT C also affects ryenctic meclTanisms in ff. coli exerting a Inutaoenic action, incrcasing tlTe recoinlJinatioiT rate (Szybalski, 1958 ; Tillin a and Hagiwara, 1960 ;Yuki, 1962), and inducing IJhaue development in a Iysouenic strain of f. co!I A' 12 (01, u, at "!., 1959).This coininunication presents evidence that ITTitomycin C can induce colicin production in colicinooenic strains of E. coli.Preliminary results have been reported elsewhere.(1111ma, 1961).