In Situ Probing of Newly Synthesized Peptidoglycan in Live Bacteria with Fluorescent <scp>D</scp>‐Amino Acids

Abstract

Unter Beobachtung: Die Toleranz von Zellen für den Einbau unterschiedlicher nichtnatürlicher D-Aminosäuren wird genutzt, um Peptidoglycan (PG) in verschiedenen Bakterien zu markieren. Die nichttoxischen D-Aminosäuren markieren bevorzugt die Orte aktiver PG-Synthese und ermöglichen so die zeitliche und räumliche Beobachtung der Zellwand-Dynamik in Bakterien. HCC=7-Hydroxycumarin, NBD=7-Nitrobenzofurazan, TAMRA=Carboxytetramethylrhodamin. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.