Observations on the changes produced in the blood and bone marrow by hæmorrhage and blood destruction. (Part II.)

Abstract

IN a previous paper2 attention was directed t o the changes seen in the blood and bone inarrow of rabbits after hteniorrhage.The preseut research was undertaken in order to study these changes more fully, and to contrast them with the changes following blood destruction by phenylhydrazin, and to consider more especially whether the fact that the remnants of red cells are left in the body produces any variation in the type of red cell regeneration from that which is followed after hzmorrhage.The megaloblast8ic marrow and megalocytic blood which are the essential characteristics of pernicious anamia are Etrongly contrasted with the low colour index of post-hzmorrhagic anzmia, and they have been looked upon by Hunter (1909 1) and others as the effect rather than the cause of the pronounced blood destruction which occurs in that disease.On this hypothesis regeneration in the marrow may be presumed to be stiniulated by the products of red cell destruction.Bunting (1 9 0 6 2, has noted the niegaloblastic response to ricin, and Yaton and Goodall (1903 3) and Kamminer (1906 4, to phenylliyclrazin, in rabbits.References to the literature of the action of phenylhydrazin on blood will be found in Itanii (1908 5), Ritz (1 9 0 9 ">, Lewin ( 1 9 0 1 '), Heinz (1 9 0 1 s), Rforawitz and Pratt ( 1 9 0 S g), and Rosenthal (1 9 10 lo). I. METHODS EMPLOYED.Estimations of hauoglobin were made with a Gowers-Haldane standardised ~iaemoglobinometer.Enumerations of the red and white blood cells mere made with Burker's modification of the Thoma-Zeiss hsmocytometer.The diluting solution used in the earlier observations was Toisson's fluid ; it was, Towards the expenses of this research a grant was made by