Spectral Absorption of Bilirubin. Messurements in Pure Aqueous Solutions and in Solutions Containing Human Serum

Abstract

Summary. Spectral absorption curves of aqueous bilirubin solutions with and without the addition of serum are given. Readings in the Pulfrich photometer showed good agreement with the spectrographic measurements. Solutions containing serum showed a maximum at 455 mμ, pure solutions at 420 mμ. The formation of a bilirubin‐serum albumin compound is the cause of the change in the spectral absorption. Values for E 1% 1cm are given.