Molecular and Cellular Biology · 1986 · 41 citations · 39 references
Life SciencesGeneticsMolecular BiologyGene ExpressionTranscriptionTranscriptional RegulationCore Promoter ElementsPromoter RecognitionPromoter RegulationTranscription FactorsTranscription InitiationTranscriptional ControlDistal Promoter ElementsGene Promoter RegulationPromoter Region IdentificationPromoter Regulatory ElementsPromotersRna Polymerase Ii ActivationTranscription Factor Binding At PromotersTranscription Factor Binding Sites
We used a cell-free HeLa cell transcription system to identify and characterize transcription factors and the promoter elements that they recognize in RNA polymerase II-transcribed genes. Deletion of the region (-71 to -83) containing the GTCAAA direct repeat resulted in a marked decrease of specific transcription of the ovalbumin gene; transcription could be competed with DNA fragments containing this sequence. Furthermore, DNase I footprinting identified a protein-binding site including this direct repeat with crude extracts and one of the partially purified protein fractions required for transcription. We propose that a soluble factor activates transcription through binding to the direct repeat of GTCAAA sequence upstream from the ovalbumin gene.
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