Temperature‐dependent Raman and infrared spectra of nucleosides. III—deoxycytidine

Abstract

Abstract Raman and infrared spectra were recorded for crystalline deoxycytidine samples at 300, 200, 100 and 10 K. Data were taken over the spectral range 20–4000 cm −1 . The wavenumbers of most vibrational modes were found to increase as the temperature was lowered. A number of peaks split into multiplets at low temperature. A new mode, observed at 375 cm −1 , is a marker band for 2 ′ ‐deoxyribose in cytosine‐containing nucleic acids. Evidence is presented supporting the assignment of marker bands associated with the C3 ′ ‐ endo and C2 ′ ‐ endo puckers of deoxyribose. No clear indication was found of any structural phase transitions in this temperature range. Several hydrogen‐stretching modes were found to involve hydrogen bonding. Plausible assignments of all the main features observed are suggested. Copyright © 2003 John Wiley & Sons, Ltd.