Rapid High‐Specificity microRNA Detection Using a Two‐stage Isotachophoresis Assay

Giancarlo Garcia‐Schwarz, Juan G. Santiago

Angewandte Chemie International Edition · 2013 · 58 citations · 25 references

DOIFull text

Open access

Concepts

Abstract

Focusing in on the small: A two-stage microRNA detection assay uses electrokinetic focusing to speed up hybridization and a functionalized hydrogel for affinity purification. This method detects microRNAs in 15 minutes with single-nucleotide specificity, and processes only 5 ng of total RNA. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.

References

25