Applied Microbiology · 1973 · 19 citations · 0 references
AgricultureFood ScienceImmunologyDiagnostic MicrobiologyFood MicrobiologyImmunotechnologyBacterial ToxinsFoodborne PathogensImmunoassay AnalysisMicrobial Toxins In FoodNatural Toxins In FoodPathogen Detection In FoodsAnalytical Methods For Food ToxinsAssayAssay SpecificityBacterial Toxin AssaysCompetitive InhibitionInterferenceStaphylococcal EnterotoxinsToxin Detection Assays
A rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A is described. The assay procedure requires 3 to 4 h for completion by using a competitive inhibition system in which the antibody is attached to bromacetyl cellulose particles. It is accurate to a level of 0.01 μg of enterotoxin A/ml in a variety of media such as ham, milk products, crab meat, custard, etc. No significant interference was found with any media or food product tested.