A Heteronuclear Direct‐Detection NMR Spectroscopy Experiment for Protein‐Backbone Assignment

Abstract

The starting point of any standard protocol for protein-structure determination by NMR spectroscopy is the sequence-specific resonance assignment of the polypeptide chain. An experiment for determining backbone assignment starts and ends on 13C nuclei and exploits only heteronuclei. This experiment paves a new route for those cases in which the 1H lines are too severely broadened.