Directional cloning of native PCR products with preformed sticky ends (Autosticky PCR)

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

Randall K. Saiki, David H. Gelfand, Susanne Stoffel et al. · Science · 1988 · 17.1K citations

Engineering, Dna Analysis, Molecular Biology +19

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

Kary B. Mullis, Fred Faloona · Methods in enzymology on CD-ROM/Methods in enzymology · 1987 · 5.7K citations

Biosynthesis, Nucleic Acid Chemistry, Biochemistry +6

Ligation-independent cloning of PCR products (LIC-PCR)

Charalampos Aslanidis, Pieter J. de Jong · Nucleic Acids Research · 1990 · 1.2K citations · Full text

Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products

Douglas A. Marchuk, Mitchell L. Drumm, Ann M. Saulino et al. · Nucleic Acids Research · 1991 · 1.2K citations · Full text

Unmodified Pcr Products, Engineering, Direct Cloning +13

Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases

James M. Clark · Nucleic Acids Research · 1988 · 842 citations · Full text

Nucleic Acid Chemistry, Biochemistry, Natural Sciences +11