The Effect of Thermal Stress on the Physiology and Bacterial Communities of Two Key Mediterranean Gorgonians

Abstract

Gorgonians are important habitat-providing species in the Mediterranean Sea, but their populations are declining due to microbial diseases and repeated mass mortality events caused by summer heat waves. Elevated seawater temperatures may impact the stress tolerance and disease resistance of gorgonians and lead to disturbances in their microbiota. However, our knowledge of the biological response of the gorgonian holobiont (<i>i.e.,</i> the host and its microbiota) to thermal stress remains limited. Here, we investigated how the holobiont of two gorgonian species (<i>Paramuricea clavata</i> and <i>Eunicella cavolini</i>) are affected throughout a 7-week thermal stress event by following both the corals' physiology and the composition of their bacterial communities. We found that <i>P. clavata</i> was more sensitive to elevated seawater temperatures than <i>E. cavolini</i>, showing a greater loss in energy reserves, reduced feeding ability, and partial mortality. This lower thermotolerance may be linked to the ∼20× lower antioxidant defense capacity in <i>P. clavata</i> compared with <i>E. cavolini</i>. In the first 4 weeks of thermal stress, we also observed minor shifts in the microbiota of both species, suggesting that the microbiota likely plays a limited role in thermal acclimation of the holobiont. However, major stochastic changes occurred later on in some colonies, which were of a transient nature in <i>E. cavolini</i>, but were linked to partial colony mortality in <i>P. clavata</i>. Overall, our results show significant, but differential, effects of thermal stress on the holobionts of both <i>E. cavolini</i> and <i>P. clavata</i> and predict potentially severe impacts on gorgonian populations under future climate scenarios. <b>IMPORTANCE</b> In the Mediterranean Sea, the tree-shaped gorgonian corals form large forests that provide a place to live for many species. Because of this important ecological role, it is crucial to understand how common habitat-forming gorgonians, like <i>Eunicella cavolini</i> and <i>Paramuricea clavata</i>, are affected by high seawater temperatures that are expected in the future due to climate change. We found that both species lost biomass, but <i>P. clavata</i> was more affected, being also unable to feed and showing signs of mortality. The microbiota of both gorgonians also changed substantively under high temperatures. Although this could be linked to partial colony mortality in <i>P. clavata</i>, the changes were temporary in <i>E. cavolini.</i> The overall higher resistance of <i>E. cavolini</i> may be related to its much higher antioxidant defense levels than <i>P. clavata.</i> Climate change may thus have severe impacts on gorgonian populations and the habitats they provide.