Genome-Wide Identification of DNA Binding with One Finger (Dof) Gene Family in Tartary Buckwheat (Fagopyrum tataricum) and Analysis of Its Expression Pattern after Exogenous Hormone Stimulation

Abstract

DNA binding with one finger (<i>Dof</i>) proteins have been proven to be involved in multiple biological processes. However, genome-wide identification of the <i>Dof</i> gene family has not been reported for Tartary buckwheat (<i>Fagopyrum tataricum</i>). In this study, 35 <i>Ft</i>Dof proteins were identified, and they could be divided into nine phylogenetic subgroups. Proteins within the same subgroup had similar gene structure and motif composition. Moreover, abundant <i>cis</i>-acting elements were present in the promoter regions of <i>FtDof</i> genes. Segmental duplication was the primary driving force for the evolution of the <i>Ft</i>Dof gene family. Synteny analysis indicated that Tartary buckwheat was closer to dicotyledons, and more orthologous <i>Dof</i> genes existed among them. The expression pattern of <i>FtDofs</i> in different tissues and at different fruit developmental stages varied. Different tissues contained several genes that were specifically expressed. <i>FtDof</i> expression was mainly upregulated under methyl jasmonate treatment and downregulated under other hormone treatments. Taken together, <i>FtDofs</i> may play important roles in the growth and development of Tartary buckwheat and in response to abiotic and biotic stresses. Therefore, the genome-wide identification and expression pattern analysis of the Tartary buckwheat <i>Dof</i> gene family lays a foundation for further exploration of the functional characteristics of <i>FtDofs</i> in the future.