Journal of the American Veterinary Medical Association · 1976 · 51 citations · 0 references
Diagnostic VirologySummary FourAnimal SciencePathogenesisAnimal HealthVeterinary SciencePathologyVirologyCell CultureEducationCulture FluidVeterinary MicrobiologyMicrobiologyMedicineHeat TreatmentAnimal Virus
SUMMARY Four sheep inoculated with mixtures of bovine leukemia virus ( blv ) and milk that was treated by a simulated high-temperature short-time pasteurization procedure did not become infected with blv or develop tumor. Four sheep inoculated with unpasteurized blv -milk mixtures became infected with blv and 3 died with tumor at 15, 21, and 27 months, respectively, after inoculation. Fluid from a blv -infected cell culture was heated to 56, 60, 65, and 73 C for ½ minute and 1 minute (in separate trials) and transferred to noninfected cells. Culture fluid heated to 60 C and higher did not infect the cells.