RESEARCHES REGARDING THE ISOLATION, PURIFICATION AND ANALYSIS OF SINIGRIN GLUCOSINOLATE FROM BRASSICA NIGRA AND ARMORACIA RUSTICANA

Abstract

The objectives of research activities aimed natural substances with antibiotic activity, come from spontaneous flora (horseradish and black mustard), which used in the shape of standardized extracts as food additives, to replace in a near future, the compounds obtained through chemical synthesis. Such compound is glugosinolate sinigrin, an exceptional source of enzymatic inductors with active microbiologic role. 
 In various temperature conditions (65-100°C),various solvents extraction (distillated water, methanol, ethanol, mixture: ethanol:water (1:1), methanol:water (1:1), purification by ionic exchange chromathography (DEAE Sephadex A-50), extraction time: 3-4 hours, a sinigrin purity of 98.01-99.11% and an efficiency of 82.85-83.62%, respectively has been obtained.