A Combined Spectrophotometer and Fluorometer to Demonstrate the Principles of Absorption Spectroscopy

Abstract

A dual-function student-crafted instrument is described as part of a laboratory activity aimed to teach both the principles and practical aspects of absorption spectroscopy to secondary and introductory undergraduate students. Using minimal changes in an arrangement that is based on interlocking bricks and low-cost components, both a fluorometer and photometer have been constructed. The former demonstrates the principles of the Beer–Lambert law visually and quantitatively by acquiring the spatial light attenuation through a fluorescent sample. The latter then demonstrates its practical application in a visible-light spectrometer by measuring the absorption spectrum of an aqueous permanganate solution.