Introduction to differential gene expression analysis using RNA-seq

Friederike Dündar, Luce Skrabanek, Paul Zumbo

Zenodo (CERN European Organization for Nuclear Research) · 2015 · 16 citations · 0 references

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Abstract

Comprehensive introduction to the processing and analysis of bulk RNA-seq data including basic information about Illumina-based short read sequencing, common file formats (FASTQ, SAM/BAM, BED, ...) and quality controls. Contains ready-to-use UNIX and R code; covers the most common application of bulk RNA-seq to identify genes that are differentially expressed when comparing two conditions.