Development of an Amplicon-Based Next-Generation Sequencing Protocol to Identify <i>Leishmania</i> Species and Other Trypanosomatids in Leishmaniasis Endemic Areas

Abstract

species, thus promoting the appearance of coinfection events, particularly in disease-endemic regions. The present study describes the application of an amplicon-based next-generation sequencing (NGS) assay to detect and identify trypanosomatid species in mammalian reservoirs, human patients, and sand fly vectors from regions of endemicity for leishmaniasis. This changes our understanding of the clinical course of leishmaniasis in areas of endemicity.