Investigation of the chemical characteristics and anticancer effect of plasma‐activated water: The effect of liquid temperature

Abstract

Abstract The liquid temperature during the preparation of plasma‐activated water (PAW) seriously affects the PAW chemical characteristics and its biological effect. In this study, four different temperatures (4°C, 25°C, 40°C, and 70°C) of deionized water are selected as variable parameters to investigate the effect on PAW. The results show that the physicochemical properties and the concentration of reactive oxygen and nitrogen species of PAW are significantly reduced when the temperatures are higher than 25°C; moreover, the above indexes are slightly decreased when the temperature is down to 4°C. Furthermore, by comparing the results of cell viability and apoptosis, it is found that the PAW of 25°C has a higher inactivation efficiency than other temperatures. Research on the temperature effect during PAW production is beneficial for tailoring the plasma–liquid system to enhance production efficiency.