ACS Omega · 2021 · 16 citations · 22 references
AgricultureFood ScienceMicrobiologyAnalytical ChemistryFood MicrobiologyNanobiotechnologyAptamer-based BiosensorsFoodborne Pathogen DetectionMagnetic SeparationNanomaterial-based BiosensorsPathogen DetectionPathogen Detection In FoodsAptamer-based BiosensingAptamer-based DetectionAptamer-based NanobiosensorsChemiluminescent BiosensorsChemiluminescent Signal AmplificationGold Nanoparticle DetectionMagnetic Nanoparticle BiosensorsMagnetic Nanoparticles
A capture probe was constructed using a combination of magnetic Fe3O4 nanoparticles and an aptamer directed towardListeria monocytogenes. A signal probe was prepared by combining luminol-functionalized flowerlike gold nanoparticles, obtained by combining luminol with chitosan bearing a complementary sequence of the aptamer. The complex consisting of the capture probe and signal probe could be removed through magnetic separation. Where the target was present within a sample, it competed with the complementary sequence for binding to the aptamer, causing a change of the chemiluminescent signal. The results indicated that a good linear relationship existed over the concentration range 1.0 × 101–1.0 × 105 CFU·mL–1. It was established that it was feasible to use this approach to detect L. monocytogenes at levels as low as 6 CFU·mL–1 in milk samples.
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Gold Nanoparticle-Catalyzed Luminol Chemiluminescence and Its Analytical Applications
Zhifeng Zhang, Hua Cui, Chun‐Ze Lai et al. · Analytical Chemistry · 2005 · 501 citations
An aptamer-based electrochemical biosensor for the detection of Salmonella
Xiaoyuan Ma, Yihui Jiang, Fei Jia et al. · Journal of Microbiological Methods · 2014 · 224 citations