Combining Kinetic and Constraint-Based Modelling to Better Understand Metabolism Dynamics

Abstract

To understand the phenotypic capabilities of organisms, it is useful to characterise cellular metabolism through the analysis of its pathways. Dynamic mathematical modelling of metabolic networks is of high interest as it provides the time evolution of the metabolic components. However, it also has limitations, such as the necessary mechanistic details and kinetic parameters are not always available. On the other hand, large metabolic networks exhibit a complex topological structure which can be studied rather efficiently in their stationary regime by constraint-based methods. These methods produce useful predictions on pathway operations. In this review, we present both modelling techniques and we show how they bring complementary views of metabolism. In particular, we show on a simple example how both approaches can be used in conjunction to shed some light on the dynamics of metabolic networks.