How a sex pheromone might act at a concentration below 10(−16) M.

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

Michael A. Frohman, Michael Dush, Gail R. Martin · Proceedings of the National Academy of Sciences · 1988 · 4.6K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +13

Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes

Dayle A. Hager, Richard R. Burgess · Analytical Biochemistry · 1980 · 1.2K citations

Life Sciences, Biochemistry, Molecular Biology +13

Anhydrous hydrogen fluoride deglycosylates glycoproteins

Andrew J. Mort, Derek T. A. Lamport · Analytical Biochemistry · 1977 · 380 citations

Life Sciences, Biochemistry, Chemistry +12

Purification and Characterization of the Hormone Initiating Sexual Morphogenesis in <i>Volvox carteri</i> f. <i>nagariensis</i> Iyengar

Richard C. Starr, Lothar Jaenicke · Proceedings of the National Academy of Sciences · 1974 · 170 citations · Full text

Life Sciences, Biochemistry, Developmental Biology +16