IL-10 Deficiency Accelerates Type 1 Diabetes Development via Modulation of Innate and Adaptive Immune Cells and Gut Microbiota in BDC2.5 NOD Mice

Abstract

Type 1 diabetes is an autoimmune disease caused by T cell-mediated destruction of insulin-producing β cells. <i>BDC2.5</i> T cells in <i>BDC2.5</i> CD4⁺ T cell receptor transgenic Non-Obese Diabetic (NOD) mice (<i>BDC2.5</i>⁺ NOD mice) can abruptly invade the pancreatic islets resulting in severe insulitis that progresses rapidly but rarely leads to spontaneous diabetes. This prevention of diabetes is mediated by T regulatory (Treg) cells in these mice. In this study, we investigated the role of interleukin 10 (IL-10) in the inhibition of diabetes in <i>BDC2.5</i>⁺ NOD mice by generating <i>Il-10</i>-deficient <i>BDC2.5</i>⁺ NOD mice (<i>BDC2.5</i>⁺<i>Il-10</i>^-/- NOD mice). Our results showed that <i>BDC2.5</i>⁺<i>Il-10</i>^-/- NOD mice displayed robust and accelerated diabetes development. <i>Il-10</i> deficiency in <i>BDC2.5</i>⁺ NOD mice promoted the generation of neutrophils in the bone marrow and increased the proportions of neutrophils in the periphery (blood, spleen, and islets), accompanied by altered intestinal immunity and gut microbiota composition. <i>In vitro</i> studies showed that the gut microbiota from <i>BDC2.5</i>⁺<i>Il-10</i>^-/- NOD mice can expand neutrophil populations. Moreover, <i>in vivo</i> studies demonstrated that the depletion of endogenous gut microbiota by antibiotic treatment decreased the proportion of neutrophils. Although <i>Il-10</i> deficiency in <i>BDC2.5</i>⁺ NOD mice had no obvious effects on the proportion and function of Treg cells, it affected the immune response and activation of CD4⁺ T cells. Moreover, the pathogenicity of CD4⁺ T cells was much increased, and this significantly accelerated the development of diabetes when these CD4⁺ T cells were transferred into immune-deficient NOD mice. Our study provides novel insights into the role of IL-10 in the modulation of neutrophils and CD4⁺ T cells in <i>BDC2.5</i>⁺ NOD mice, and suggests important crosstalk between gut microbiota and neutrophils in type 1 diabetes development.