Studies on Continuous Enzyme Reactions

Abstract

The kinetics of hydrolysis of acetyl-DL-methionine in DEAE-cellulose-aminoacylase (EC 3. 5. 1. 14) column and DEAE-Sephadex-aminoacylase column was studied. The rate of hydrolysis of substrate was shown to be dependent on the flow rate and independent to the dimension of the enzyme column. The rate of hydrolysis of the substrate was equal in cases of down-waard flow and of up-ward flow. The deteriorated aminoacylase columns by long period operation were reactivated by the recharge of aminoacylase to them. The continuous enzyme reaction using an aminoacylase column was superior to the batch enzyme reaction using native aminoacylase.