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A new method of recovering polyhydroxyalkanoate from Azotobacter chroococcum
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2000
Year
Non-pha BiomassBiomanufacturingEngineeringPha ContentBiorefinery ProductBiochemical EngineeringBiotechnologyAzotobacter ChroococcumAlgal ProductDownstream ProcessingEnvironmental MicrobiologyPha PelletMicrobiologyMedicineMicrobiological Degradation
After polyhydroxyalkanoate (PHA) fermentation for 42 - 48 h by the Azotobacter chroococcum G-3, the PHA content reached more than 75% of the dry weight. Biomass was isolated from culture by centrifugation and pretreated with freezing to release PHA pellet, then was treated with 10 g/L sodium dodecyl sulfate (SDS) for 15 min to effectively solubilize lipid and protein. Subsequently, it was further purified by digesting with 30% sodium hypochlorite (NaCIO) for 3 min to remove peptidoglycan and non-PHA biomass. Finally, 98% PHA was obtained by diluting and rinsing with water, and the PHA recovered was suitable for processing.