A low-cost 3D printed microfluidic bioreactor and imaging chamber for live-organoid imaging

Abstract

Organoids are biological systems grown <i>in vitro</i> and are observed to self-organize into 3D cellular tissues of specific organs. Brain organoids have emerged as valuable models for the study of human brain development in health and disease. Researchers are now in need of improved culturing and imaging tools to capture the <i>in vitro</i> dynamics of development processes in the brain. Here, we describe the design of a microfluidic chip and bioreactor, to enable <i>in situ</i> tracking and imaging of brain organoids on-chip. The low-cost 3D printed microfluidic bioreactor supports organoid growth and provides an optimal imaging chamber for live-organoid imaging, with drug delivery support. This fully isolated design of a live-cell imaging and culturing platform enables long-term live-imaging of the intact live brain organoids as it grows. We can thus analyze their self-organization in a controlled environment with high temporal and spatial resolution.