Evaluation of anticarcinogenic and antioxidant properties of Eruca sativa extracts versus Ehrlich ascites carcinoma in mice

Abstract

Cancer is a major public health issue and characterized by wild cell growth and proliferative disorder. The antitumor and antioxidant role of Eruca sativa (ES) was investigated in a Ehrlich ascites carcinoma (EAC) tumor model in mice. Twenty-four hours after EAC intraperitoneal inoculation in mice, ES extracts were administered at doses of 250 mg/kg/body weight (BW) for 9 days. Half of the mice were sacrificed on the 10th day for estimation of hematological profiles, red blood cell count, hemoglobin content, packed cell volume, mean corpuscular volume and mean corpuscular hemoglobin. In addition, antioxidant parameters (superoxide dismutase, catalase, and glutathione peroxidase) were evaluated. The remaining mice were kept alive to assess the increase in life span. Aspartate aminotransferase, alanine transaminase, alkaline phosphatase, creatinine, and urea levels were assessed for ES antitumor effects. Results. Oral supplementation of ES seed and leaf extracts significantly reduced tumor parameters such as tumor volume and tumor weight with remarkable enhance in tumor inhibition ratio, vice versa, increased both survival and average BW of tumor-bearing mice. ES extracts significantly increased BW and extended the life span of tumor-bearing mice. Hematological profiles were restored to normal and modulated the abovementioned antioxidant parameters in ES-treated mice compared with EAC control. The current study noticeably demonstrates the antitumor activity of ES seed and leaf extracts significantly prolonged life span and normalized the hematological profiles and biochemical parameters compared with EAC mice.