Mesoporous silica nanoparticles functionalized with folic acid for targeted release Cis-Pt to glioblastoma cells

Abstract

Abstract This work reports the preparation, characterization, and a drug release study of mesoporous silica nanoparticles (MNPSiO 2 ) functionalized with folic acid (FA) and loaded with Cis-Pt as a targeted release system to kill glioblastoma cancer cells. The MNPSiO 2 were synthesized by the Stöber method using hexadecyltrimethylammonium bromide as the templating agent, which was finally removed by calcination at 550°C. The folic acid was chemically anchored to the silica nanoparticles surface by a carbodiimide reaction. Several physicochemical techniques were used for the MNPSiO 2 characterization, and a triplicate in vitro Cis-Pt release test was carried out. The release Cis-Pt experimental values were fitted to different theoretical models to find the Cis-Pt release mechanism. The cytotoxicity evaluation of the MNPSiO 2 was performed using LN 18 cells (human GBM cells). Homogeneous and well-defined nanoparticles with well-distributed and homogeneous porosity were obtained. The spectroscopic results show the proper functionalization of the mesoporous nanoparticles; besides, MNPSiO 2 showed high surface area and large pore size. High correlation coefficients were obtained. Though the best fitted was the Korsmeyer-Peppas kinetic model, the Higuchi model adjusted better to the results obtained for our system. The MNPSiO 2 -FA were highly biocompatible, and they increased the cytotoxic effect of Cis-Pt loaded in them.