Improvement of adventitious root formation in Sedum aizoon L. and the production of flavonoids

Abstract

• 2.0 mg•L −1 2,4-D/0.5 mg•L −1 6-BA is needed to callus induction in Sedum aizoon L . • 3 mg•L −1 IBA is suitable for induction of adventitious roots in Sedum aizoon L. • IBA, NAA and KT are necessary for adventitious roots induction in Sedum aizoon L. • Stratified combination medium is more helpful for the adventitious roots. • Induced adventitious roots of Sedum aizoon L. can be used to produce flavonoids. Sedum aizoon L. is a medicinal plant that contains various flavonoids and phenolic acids, and plays positive roles in lowering blood pressure, hemostasis, anti-bacterial and anti-inflammatory. However, the clinical application of Sedum aizoon L. is limited because of its low yield in natural state. Here, we aim to explore the application of adventitious root culture technology in Sedum aizoon L., further to improve the yield of Sedum aizoon L. and the production of secondary metabolites. We found that the callus of Sedum aizoon L. could be induced in MS medium containing 2.0 mg L −1 2,4-D and 0.5 mg L −1 BA. Then, the adventitious roots were obtained in Sedum aizoon L . with maximum induction rate (98.32%) when the callus were cultured in a stratified combination MS medium supplemented with 4.0 mg L −1 IBA, 1.0 mg L −1 KT, 1.0 mg·L −1 NAA and 60 mmol L −1 NO 3 − /NH 4 + (5:1 ratio). Furthermore, the production of flavonoids in adventitious roots of S. aizoon L. (16.32±0.51 mg·g −1 DW) was obviously higher than those in the callus (3.66±0.65 mg g −1 DW). In conclusion, we determined that adventitious roots culture might be an effective alternative in improving the potential application of Sedum aizoon L . .