A potential endogenous gibberellin-mediated signaling cascade regulated floral transition in Magnolia × soulangeana ‘Changchun’

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

Kenneth J. Livak, Thomas D. Schmittgen · Methods · 2001 · 181.5K citations

Life Sciences, Molecular Biology, Molecular Genetics +9

Trimmomatic: a flexible trimmer for Illumina sequence data

Anthony Bolger, Marc Lohse, Björn Usadel · Bioinformatics · 2014 · 69.7K citations · Full text

Life Sciences, Bioinformatics, Molecular Biology +13

Fast gapped-read alignment with Bowtie 2

Ben Langmead, Steven L. Salzberg · Nature Methods · 2012 · 61.1K citations

Life Sciences, Bioinformatics, Molecular Biology +11

BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs

Felipe A. Simão, Robert M. Waterhouse, Panagiotis Ioannidis et al. · Bioinformatics · 2015 · 14.5K citations · Full text

Life Sciences, Bioinformatics, Genetics +17

De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis

Brian J. Haas, Alexie Papanicolaou, Moran Yassour et al. · Nature Protocols · 2013 · 9.1K citations · Full text

Life Sciences, Bioinformatics, Molecular Biology +16