A hepatocyte-targeting fluorescent probe for imaging isoniazid-induced hydrazine in HepG2 cells and zebrafish

Abstract

A hepatocyte-targeting fluorescent N₂H₄ probe, GHP, was first designed and synthesized employing N-acetylgalactosamine (GalNAc) as the hepatocyte-targeting group and 3-nitrophthalimide as the recognition moiety. The probe can be used to selectively image N₂H₄ produced by the hydrolysis of isoniazid in HepG2 cells and the liver of zebrafish in situ.