Spontaneous membrane-less multi-compartmentalization <i>via</i> aqueous two-phase separation in complex coacervate micro-droplets

Abstract

Polyelectrolyte/nucleotide multiphase complex coacervate droplets are produced by internalized aqueous two-phase separation and used for the spatially dependent chemical transfer of sugar molecules, providing a step towards the development of membrane-free "organelles" within coacervate-based protocells.