Napthrene Compounds from Mycelial Fermentation Products of Marasmius berteroi

Abstract

The metabolites of the genus <i>Marasmius</i> are diverse, showing good research prospects for finding new bioactive molecules. In order to explore the active metabolites of the fungi <i>Marasmius berteroi</i>, the deep chemical investigation on the bioactive compounds from its cultures was undertaken, which led to the isolation of three new naphthalene compounds dipolynaphthalenes A-B (<b>1</b>,<b>2</b>) and naphthone C (<b>3</b>), as well as 12 known compounds (<b>4</b>-<b>15</b>). Compounds <b>1</b>, <b>2</b>, and <b>4</b> are dimeric naphthalene compounds. Their structures were elucidated by MS, 1D and 2D NMR spectroscopic data, as well as ECD calculations. Compounds <b>2</b>-<b>4</b> and <b>7</b> exhibited acetylcholinesterase (AChE) inhibitory activities at the concentration of 50 μg/mL with inhibition ratios of 42.74%, 44.63%, 39.50% and 51.49%, respectively. Compounds <b>5</b> and <b>7</b>,<b>8</b> showed weak inhibitory activities towards two tumor cell lines, with IC₅₀ of 0.10, 0.076 and 0.058 mM (K562) and 0.13, 0.18, and 0.15 mM (SGC-7901), respectively.