Engineering Corynebacterium glutamicum with a comprehensive genomic library and phage-based vectors

One-step inactivation of chromosomal genes in <i>Escherichia coli</i> K-12 using PCR products

Kirill A. Datsenko, Barry L. Wanner · Proceedings of the National Academy of Sciences · 2000 · 15.2K citations · Full text

Life Sciences, Genetics, Molecular Genetics +17

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations

Life Sciences, Microbiology, Molecular Biology +11

Studies on transformation of Escherichia coli with plasmids

Douglas Hanahan · Journal of Molecular Biology · 1983 · 11.3K citations

Life Sciences, Genetics, Microbiology +12

The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential applications

Paul M. Sharp, Wen‐Hsiung Li · Nucleic Acids Research · 1987 · 4.1K citations · Full text

Life Sciences, Genetics, Molecular Genetics +15

Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum

Andreas Schäfer, Andreas Tauch, Wolfgang Jäger et al. · Gene · 1994 · 2.8K citations

Life Sciences, Genetics, Microbiology +17