Comparative transcriptomic analysis of <i>Lactiplantibacillus plantarum</i> RS66CD biofilm in high-salt conditions and planktonic cells

Abstract

<i>L. plantarum</i> RS66CD biofilm formation formed highest amount of when grown at 48 g L^-1 NaCl. Altogether 447 genes were up-regulated and 426 genes were down-regulated in the biofilm. KEGG pathway analysis showed that genes coding for D-Alanine metabolism, peptidoglycan biosynthesis, two-component system, carbon metabolism, bacterial secretion system, lysine biosynthesis and fatty acid metabolism were crucial for biofilm formation. In addition, eight other genes related to biofilm formation were differentially expressed. Our results provide insights into the differential gene expression involved in biofilm formation, which can help to reveal gene regulation during <i>L. plantarum</i> biofilm formation.