Chemical Communications · 2020 · 220 citations · 15 references
Life SciencesInfectious DiseasesMolecular BiologyMolecular DiagnosticsMolecular VirologyVirologyAptamer-based BiosensorsDna BiosensorsHost-pathogen RecognitionInfectious Disease Molecular TestingPathogen DetectionViral DiagnosticsAptamer DesignAptamer DiscoveryAptamer RecognitionAptamer-based AssaysAptamer-based BiosensingAptamer-based DetectionAptamer-based ProbesAptamers
value of 0.49 nM. Interestingly, we found that the four pairs of aptamers could bind to Np successively, suggesting a sandwich-type interaction. Using these sandwiched aptamers in ELISA and colloidal gold immunochromatographic strips, we were able to detect Np at the tens of pM level. The results demonstrate that aptamers are powerful molecular tools for virus detection, diagnosis, and antiviral therapy.
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