Gordonia mangrovi sp. nov., a novel actinobacterium isolated from mangrove soil in Hainan

Abstract

A novel actinobacterium, designated strain HNM0687 T , was isolated from mangrove soil samples collected from Hainan Province, PR China and its polyphasic taxonomy was studied. Based on the results of 16S rRNA gene sequence analysis, strain HNM0687 T was closely related to Gordonia bronchialis NBRC 16047 T (98.7 %), Gordonia rhizosphera NBRC 16068 T (98.2 %), Gordonia oryzae RS15-1S T (97.9 %), Gordonia polyisoprenivorans NBRC 16320 T (97.7 %) and Gordonia sediminis AMA 120 T (97.7 %). Genome-based comparisons revealed a clear distinction in average nucleotide identity values between strain HNM0687 T and its closely related strains (74.4–78.3 %). Strain HNM0687 T contained meso -diaminopimelic acid, arabinose and galactose in whole-cell hydrolysates. Mycolic acid was present. The menaquinones of strain HNM0687 T were MK-9(H 2 ) and MK-7(H 2 ). The phospholipids of the isolate were composed of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were C 16 : 0 , C 16 : 1 ω 7 c /C 16 : 1 ω 6 c , C 18 : 0 10-methyl (TBSA), C 18 : 0 and C 18 : 1 ω 9 c . Based on its genotypic, chemotaxonomic and phenotypic characteristics, it is concluded that strain HNM0687 T represents a novel species of the genus Gordonia for which the name Gordonia mangrovi sp. nov. is proposed. The type strain is HNM0687 T (=CCTCC AA 2019074 T =KCTC 49383 T ).