FORMULATION AND CHARACTERIZATION OF QUERCITRIN NANOEMULSION ISOLATED FROM Dendropthoe falcata AND ITS ANTIOXIDANT ACTIVITY TEST

Abstract

Quercitrin is the main component that can be isolated from the parasitic plant Dendrophthoe falcata and exhibits high antioxidant activity. The purpose of this study is to make a quercitrin nanoemulsion formulation and test its antioxidant activity. This research method uses spontaneous emulsion. Quercitrin is formulated by spontaneous emulsion with the addition of oil, tween 80 as a surfactant, and phosphate buffer pH 7.0 as a water phase at low temperatures. Quercitrin nanoemulsion products are further characterized which include particle size, polydispersity index, zeta potential, physical properties, and stability. The antioxidant activity using the DPPH (2,2-diphenyl-1picrylhydrazyl) method. The results showed that quercitrin nanoemulsion had an average particle size diameter of 10.2 to 36.5 nm, polydispersity index (PI) of less than 0.5, and zeta potential of -1.8 to -11.9 mV. Quercitrin nanoemulsion using virgine coconut oil (VCO) showed better stability than olive oil. The morphology of quercitrin nanoemulsion has the shape of a spherical particle. Quercitrin nanoemulsion is easily soluble in water and exhibits very high antioxidant activity like the original quercitrin.