Lathyrane diterpenoids from <i>Jatropha podagrica</i> and their antitumor activities in human osteosarcoma cells

Abstract

Two new lathyrane-type diterpenoids, jatropodagins A and B (<b>1</b> and <b>2</b>), and five known analogues (<b>3</b>-<b>7</b>), were isolated from the stems of <i>Jatropha podagrica</i>. Their structures and absolute configurations were elucidated by spectroscopic data and calculated ECD analyses. The cytotoxicities of all the lathyrane-type diterpenoids (<b>1</b>-<b>7</b>) were evaluated against two human osteosarcoma cell lines (Saos-2 and MG-63). Compound <b>1</b> exhibited significant cytotoxic effects against Saos-2 and MG-63 with IC₅₀ values of 8.08 and 14.64 <i>μ</i>M, respectively. The IC₅₀ values for the positive control 5-FU against the Saos-2 and MG-63 cell lines were 19.01 and 25.00 <i>μ</i>M, respectively. Morphological features of apoptosis activities were evaluated in <b>1</b>-treated Saos-2 cells and the results confirmed apoptosis in a dose-dependent manner.