Concepedia

Bradford Assay for Determining Protein Concentration

Clara L. Kielkopf, William R. Bauer, Ina L. Urbatsch

Cold Spring Harbor Protocols · 2020 · 565 citations · 4 references

DOI

Concepts

Abstract

The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.

References

4

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

Mark A. Bradford · Analytical Biochemistry · 1976 · 225.4K citations

, , +7

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

Marion M. Bradford · Analytical Biochemistry · 1976 · 211.2K citations

, , +7

Increased uniformity in the response of the Coomassie blue G protein assay to different proteins

Christa M. Stoscheck · Analytical Biochemistry · 1990 · 143 citations

, , +6

Methods for Measuring the Concentrations of Proteins

Clara L. Kielkopf, William R. Bauer, Ina L. Urbatsch · Cold Spring Harbor Protocols · 2020 · 54 citations

, , +13