Tailor-made β-glucosidase with increased activity at lower temperature without loss of stability and glucose tolerance

Abstract

We evolved the thermophilic β-glucosidase <italic>Cs</italic>BglA to 150% improvement of <italic>k</italic>_cat/<italic>K</italic>_M at 55 °C, exerting a specific activity of 504 U mg⁻¹ and a <italic>K</italic>_M-value of 37.1 mM, making it the bacterial β-glucosidase with highest activity known so far.