The thermoacidophilic methanotroph <i>Methylacidiphilum fumariolicum</i> SolV oxidizes subatmospheric H2 with a high-affinity, membrane-associated [NiFe] hydrogenase

Abstract

The trace amounts (0.53 ppmv) of atmospheric hydrogen gas (H₂) can be utilized by microorganisms to persist during dormancy. This process is catalyzed by certain Actinobacteria, Acidobacteria, and Chloroflexi, and is estimated to convert 75 × 10¹² g H₂ annually, which is half of the total atmospheric H₂. This rapid atmospheric H₂ turnover is hypothesized to be catalyzed by high-affinity [NiFe] hydrogenases. However, apparent high-affinity H₂ oxidation has only been shown in whole cells, rather than for the purified enzyme. Here, we show that the membrane-associated hydrogenase from the thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV possesses a high apparent affinity (K_m(app) = 140 nM) for H₂ and that methanotrophs can oxidize subatmospheric H₂. Our findings add to the evidence that the group 1h [NiFe] hydrogenase is accountable for atmospheric H₂ oxidation and that it therefore could be a strong controlling factor in the global H₂ cycle. We show that the isolated enzyme possesses a lower affinity (K_m = 300 nM) for H₂ than the membrane-associated enzyme. Hence, the membrane association seems essential for a high affinity for H₂. The enzyme is extremely thermostable and remains folded up to 95 °C. Strain SolV is the only known organism in which the group 1h [NiFe] hydrogenase is responsible for rapid growth on H₂ as sole energy source as well as oxidation of subatmospheric H₂. The ability to conserve energy from H₂ could increase fitness of verrucomicrobial methanotrophs in geothermal ecosystems with varying CH₄ fluxes. We propose that H₂ oxidation can enhance growth of methanotrophs in aerated methane-driven ecosystems. Group 1h [NiFe] hydrogenases could therefore contribute to mitigation of global warming, since CH₄ is an important and extremely potent greenhouse gas.