Analytical Methods · 2020 · 14 citations · 15 references
EngineeringBiotechnologyMolecular BiologyBiosensorsMilk ChemistryMolecular DiagnosticsAntigen Detection AssaysAptamer-based BiosensorsCasein ChemistryMilk ProteinsMolecular SensorsOptical BiosensorsAptamer BiosensorsAptamer-based AssaysAptamer-based BiosensingAptamer-based DetectionAptamer-based SensorsFluorescent AptasensorsOptical AptasensorsProtein Detection
We present a novel, fluorogenic peptide aptamer that strongly enhances its fluorescence just after mixing it with a milk allergen α<sub>s</sub>-casein. Notably, our system can detect α<sub>s</sub>-casein in an exceptionally short time of 20–25 seconds.
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In vitro selection of RNA molecules that bind specific ligands
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Cell-free translation reconstituted with purified components
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