Neither xylem collapse, cavitation, or changing leaf conductance drive stomatal closure in wheat

Abstract

Abstract Identifying the drivers of stomatal closure and leaf damage during stress in grasses is a critical prerequisite for understanding crop resilience. Here, we investigated whether changes in stomatal conductance ( g s ) during dehydration were associated with changes in leaf hydraulic conductance ( K leaf ), xylem cavitation, xylem collapse, and leaf cell turgor in wheat ( Triticum aestivum ). During soil dehydration, the decline of g s was concomitant with declining K leaf under mild water stress. This early decline of leaf hydraulic conductance was not driven by cavitation, as the first cavitation events in leaf and stem were detected well after K leaf had declined. Xylem vessel deformation could only account for <5% of the observed decline in leaf hydraulic conductance during dehydration. Thus, we concluded that changes in the hydraulic conductance of tissues outside the xylem were responsible for the majority of K leaf decline during leaf dehydration in wheat. However, the contribution of leaf resistance to whole plant resistance was less than other tissues (<35% of whole plant resistance), and this proportion remained constant as plants dehydrated, indicating that K leaf decline during water stress was not a major driver of stomatal closure.