A continuous evolution system for contracting the host range of bacteriophage T7

One-step inactivation of chromosomal genes in <i>Escherichia coli</i> K-12 using PCR products

Kirill A. Datsenko, Barry L. Wanner · Proceedings of the National Academy of Sciences · 2000 · 15K citations · Full text

Construction of <i>Escherichia coli</i> K‐12 in‐frame, single‐gene knockout mutants: the Keio collection

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Bacteriology, Single‐gene Knockout Mutants, Molecular Biology +5

CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

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Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay

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Recombineering: a homologous recombination-based method of genetic engineering

Shyam K. Sharan, Lynn C. Thomason, Sergey G. Kuznetsov et al. · Nature Protocols · 2009 · 810 citations · Full text

Engineering, Genetics, Synthetic Biology +5