High density polyethylene (HDPE) microplastics impair development and swimming activity of Pacific oyster D-larvae, Crassostrea gigas, depending on particle size

Abstract

Understanding the effects of plastic debris on marine ecosystems is essential in encouraging decision-makers to take action. The present study investigates the effect of a 24 h experimental exposure to high density polyethylene (HDPE) microplastics (MPs) of different sizes (4-6, 11-13 and 20-25 μm) and at three concentrations (0.1, 1 and 10 mg MP.L^-1) on the development and locomotor activity of early stages of Pacific oyster, Crassostrea gigas. The bivalve embryo-larval assay (NF ISO 17244, 2015) was used in this study but with additional toxicity criteria: developmental arrests, abnormal D-larvae, maximum speed and swimming trajectory. Copper (Cu), was used as a positive control. Our results show that smaller MPs (4-6 and 11-13 μm) induced higher rates of malformations and developmental arrests than the larger ones (20-25 μm). In addition, a dose-dependent decrease of maximum swimming speed was observed for larvae exposed to MPs of 4-6 and 11-13 μm. On the other hand, there was no significant difference in swimming speed with the largest MPs size tested (20-25 μm). For all three sizes of MPs, there was a decrease in straight-line swimming trajectories, and an increase in circular trajectories. This abnormal swimming behaviour could affect larvae survival as well as colonization of new habitats.